"pub" (MEDLINE format for PubMed outputs)
PMID- 10762618
OWN - NLM
STAT- MEDLINE
DA - 20000503
DCOM- 20000503
LR - 20051117
PUBM- Print
IS - 0193-1857 (Print)
VI - 278
IP - 4
DP - 2000 Apr
TI - Upregulation of HGF activator inhibitor type 1 but not type 2 along with
regeneration of intestinal mucosa.
PG - G635-43
AB - Hepatocyte growth factor (HGF) activator inhibitor type 1 (HAI-1) and type
2 (HAI-2) are new Kunitz-type serine protease inhibitors that were
recently purified and cloned from the human stomach cancer cell line MKN45
as specific inhibitors against HGF activator. Both proteins contain two
Kunitz inhibitor domains and are expressed abundantly throughout the
gastrointestinal tract, in addition to the placenta, pancreas, and kidney.
In this study, to assess the possible roles of HAI-1 and HAI-2 in the
intestinal mucosa, we examined the expression of HAI-1 and HAI-2 during
regeneration of the intestinal mucosa. Immunohistochemical studies
revealed that HAI-1 but not HAI-2 was detected more strongly in
regenerative epithelium than in normal epithelium, although both proteins
were detected throughout the human gastrointestinal tract. During the
course of acetic acid-induced experimental colitis in an in vivo mouse
model, HAI-1 but not HAI-2 was upregulated in the recovery phase,
suggesting that HAI-1 but not HAI-2 is associated with the regeneration of
damaged colonic mucosa. Upregulation of HAI-1 may serve to downregulate
the proliferative response after initial activation of MET receptor by
HGF/scatter factor after an injury.
AD - Second Department of Pathology, Miyazaki Medical College, 5200 Kihara,
Kiyotake, Miyazaki 889-1692, Japan.
"xml"
<PMID>10762618</PMID>
<Year>2000</Year>
<ArticleTitle>Upregulation of HGF activator inhibitor type 1 but not type 2 along with regeneration of intestinal mucosa.</ArticleTitle>
<AbstractText>Hepatocyte growth factor (HGF) activator inhibitor type 1 (HAI-1) and type 2 (HAI-2) are new Kunitz-type serine protease inhibitors that were recently purified and cloned from the human stomach cancer cell line MKN45 as specific inhibitors against HGF activator. Both proteins contain two Kunitz inhibitor domains and are expressed abundantly throughout the gastrointestinal tract, in addition to the placenta, pancreas, and kidney. In this study, to assess the possible roles of HAI-1 and HAI-2 in the intestinal mucosa, we examined the expression of HAI-1 and HAI-2 during regeneration of the intestinal mucosa. Immunohistochemical studies revealed that HAI-1 but not HAI-2 was detected more strongly in regenerative epithelium than in normal epithelium, although both proteins were detected throughout the human gastrointestinal tract. During the course of acetic acid-induced experimental colitis in an in vivo mouse model, HAI-1 but not HAI-2 was upregulated in the recovery phase, suggesting that HAI-1 but not HAI-2 is associated with the regeneration of damaged colonic mucosa. Upregulation of HAI-1 may serve to downregulate the proliferative response after initial activation of MET receptor by HGF/scatter factor after an injury.</AbstractText>